Bravo, Bartra, Douglas and Sergi Roberto all miss out.
Luis Enrique has called up 18 players for the Cup semi-final second leg against Villarreal: Ter Stegen, Masip, Montoya, Piqué, Rakitic, Sergio, Xavi, Pedro, Iniesta, Suárez, Messi, Neymar Jr, Rafinha, Mascherano, Jordi Alba, Adriano, Alves and Mathieu.
Bravo, Bartra, Douglas and Sergi Roberto, as well as the injured Vermaelen all miss out on the trip.
The squad are travelling to Villarreal on Wednesday, with kick off set for 8.00 pm CET.