Spain left-back Jordi Alba will return to his club side Barcelona for further tests on a thigh injury picked up in the European champions 1-0 win over Ukraine on Friday.
Alba had to be replaced by Bayern Munich's Juan Bernat 12 minutes from time and will miss Spain's friendly in Amsterdam against the Netherlands on Tuesday.
"Our doctors have been in contact with Barcelona's doctors," said Spain boss Vicente del Bosque.
"He will leave the squad tomorrow and have tests in Barcelona to see what he has. It is a muscular injury, but we need to wait for more tests."
The injury will be of serious concern for Barcelona who are attempting to win the treble of La Liga, Copa del Rey and Champions League for only the second time in the club's history.
Barca face Paris Saint-Germain in the first leg of their Champions League quarter-final on April 15.