Manufacture
Tempe wrapped in banana leaves are sold in traditional markets Indonesia
There are various methods of making tempe. [1] [2] However, the technique of making tempe in Indonesia generally consists of the stages of boiling, peeling, soaking and pickling, washing, inoculation with yeast, packaging, and fermentation. [3]
In the early stages of making tempeh, soy beans boiled. This boiling stage serves as a hydration process, namely that of soy beans absorb as much water as possible. Boiling is also intended to soften soybeans that will be able to absorb the acid in the immersion phase.
Skin soy beans shelled on stage stripping in order to be able to penetrate the fungal mycelium soybean seeds during fermentation. Peeling can be done by hand, trampled by foot, or by seed skin peeler.
Once peeled, soaked soy beans. The goal is for the immersion phase hydration of soy beans and let the natural lactic acid fermentation in order to obtain the necessary acidity for the growth of fungi. Lactic acid fermentation occurs characterized by the emergence of a sour smell and scum on the water immersion due to the growth of Lactobacillus. When the growth of lactic acid bacteria is not optimal (eg in subtropical countries [4], the acid should be added to the soaking water, lactic acid fermentation and acidification, it is also beneficial to increase the nutritional value and eliminate toxic bacteria.
The end of the washing process carried out to remove impurities that may
The end of the washing process carried out to remove impurities that may be formed by lactic acid bacteria and soya beans so not too acidic. Bacteria and their droppings can inhibit the growth of fungi.
Inoculation is done with the addition of inoculum, ie tempeh or laru. Inoculum may be a mold that grows on leaves and dried hibiscus or teak leaves (called usar; traditionally used), tempeh mold spores in the medium flour (wheat, rice, or tapioca; sold in the market), or pure culture of R. oligosporus (commonly used by outside Indonesian tempeh maker). [4] [5] Inoculation can be done in two ways, namely (1) stocking inoculum on the surface of the soybeans are already cold and dried, then mixed evenly before wrapping; or (2) the inoculum can be mixed directly at the time of immersion, left for some time, and then dried.
Once inoculated, soy beans wrapped or placed in containers for fermentation. A variety of packaging materials or containers can be used (eg banana leaves, hibiscus leaves, leaf teak, plastic, glass, wood, and steel), provided that allows the intake of air as tempeh molds need oxygen to grow. Packing materials from the leaves or plastic is usually given by way of holes pierced.
Soybean seeds that have been wrapped allowed to undergo a process of fermentation. In this process the mold growing on the surface and penetrate the seeds of soybean, incorporate them into tempeh. Fermentation can be carried out at a temperature of 20 ° C-37 ° C for 18-36 hours. Shorter fermentation time is usually for tempeh uses many inoculum and higher temperatures, while the traditional process using laru of leaf fermentation usually takes up to 36 hours.